Journal: Water

Article Title: Effects of Underwater Lighting Time on the Growth of Vallisneria spinulosa Yan and Its Water Restoration Process

doi: 10.3390/w16243697

Figure Lengend Snippet: Figure 6. CLSM diagram of leaf–biofilm complex in different light duration ratios. (a) LV: V. spin- ulosa + low light duration ratio, (b) MV: V. spinulosa + medium light duration ratio, and (c) HV: V. spinulosa + high light duration ratio. Red is EPS polysaccharide stained with Texas red, green is protein stained with FITC, and bright blue is DNA stained with DAPI.

Article Snippet: CLSM Determination Plant leaves were collected and cut into small pieces of 5 mm × 5 mm and then placed in a 24−well microplate containing 2.5% glutaraldehyde (0.1 M PBS, pH = 7.2) phosphate buffer for 24 h. After washing 3–5 times with 0.1 M PBS solution (pH = 7.2) (Shanghai Macklin Biochemical Technology Co., Ltd., Shanghai, China), the DNA, extracellular polysaccharides, and proteins were labeled with the fluorescent dyes DAPI, Con A−Texas red conjugate solution (Invitrogen, San Diego, CA, USA), and FITC (Shanghai Macklin Biochemical Technology Co., Ltd., Shanghai, China), respectively.

Techniques: Staining